Measure or to search for specific features Box ? CH - 9436 Balgach ? Phone + 41fi71fi722fi83 33 ? Fax + 41fi71fi722fi87 58 ? THE SWISS DNA COMPANY Application Note ? Next Generation Sequencingįigure 2A ). Microsynth AG, SwitzerlandSch?tzenstrasse 15 ? P.O. Various steps dependent on the customer's requirements. The workflow can be entered and exited at Microsynth's workflow for RNA sequencing projects. Yourself or outsource this critical step toįigure 1. To include at least three biological rep. To obtain statisti -Ĭally valid results for a differential gene The use of controls as well as appropri -Īre only a few examples of points to con -īiological replicates. Process and possibilities to validate your Scriptome and sufficient numbers of bio -Įnsure a high reliability and accuracy ofįor further information on our validation Study genetic profiles from transcript to pathway levelĮrful tool to investigate the transcrip. Exemplary cut-out of a table with screening results for resistance, viru -Ĭompare gene expression profiles obtained under different conditions Sam ple _ID R esis ta n ce g e n es V ir u le n ce g e n es T o xin g e n es Priate phylogenetic context to determine its exact sequence type or its next A customer sample would be placed into its appro. Logenetic profiles stored on the PubMLST database of the Cronobacter MLST subtree: As an illustrative example, a small subtree of all phy. The html format the data are also given in tab separated format (to import into Excel) and as vcf.įigure 2. For each sample and chromosome/Ĭontig, the SNV and small InDels are reported separately and the effect of SNVs and small InDels for all annotated features of the reference genome are shown. SNV und InDels - Typical output overview file (HTML) resulting from the variant calling step for SNVs and small InDels. Ing resistance, virulence and toxin genes One or multiple variant callersĪnd reference databases (see Figure 2 ). Sible single nucleotide variations (SNVs) Refinement based on best practices, pos. Sequencing reads are quality filtered and Box ? CH - 9436 Balgach ? Phone + 41ff71ff722ff83 33 ? Fax + 41ff71ff722ff87 58 ? THE SWISS DNA COMPANY Application Note ? Next Generation Sequencing Steps depending on the requirements of the customer. The workflow can be entered and exited at various Microsynth’s workflow for resequencing projects. Ing datapackages for this service, flex -įigure 1. The experimental design, the libraries are Source the entire analysis or only single Is to provide high quality one-stop ser. This application note willīial resequencing as well as its possible Our customers state-of-the-art NGS plat -Īnalysis area. Ogies, it has never been easier to rese -Įxists). Search for virulence and resistance genes and check for mutations
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